Assign a rlog transformed assay for DEGdata object
rlg_deg.Rdrlg_deg calulate a rolg transformed assay based on rlog()
Arguments
- DEGdata
a DEGdata object from get_res
- blind
logical, whether to blind the transformation to the experimental design. blind=TRUE should be used for comparing samples in an manner unbiased by prior information on samples, for example to perform sample QA (quality assurance). blind=FALSE should be used for transforming data for downstream analysis, where the full use of the design information should be made. blind=FALSE will skip re-estimation of the dispersion trend, if this has already been calculated. If many of genes have large differences in counts due to the experimental design, it is important to set blind=FALSE for downstream analysis.
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Other options in
rlog().